Quantitative Live-cell Reporter Assay for Noncanonical Wnt Activity

A Novel Quantitative Fluorescent Reporter Assay for RAG Targets and RAG Activity

Recombination-Activating Genes (RAG) 1 and 2 form the site specific recombinase that mediates V(D)J recombination, a process of DNA editing required for lymphocyte development and responsible for their diverse repertoire of antigen receptors. Mistargeted RAG activity associates with genome alteration and is responsible for various lymphoid tumors. Moreover several non-lymphoid tumors express RA...

A fluorescent reporter of caspase activity for live imaging.

There is a growing interest in the mechanisms that control the apoptosis cascade during development and adult life. To investigate the regulatory events that trigger apoptosis in whole tissues, we have devised a genetically encoded caspase sensor that can be detected in live and fixed tissue by standard confocal microscopy. The sensor comprises two fluorophores, mRFP, monomeric red fluorescent ...

Macrophage reporter cell assay for screening immunopharmacological activity of cell wall-active antifungals.

Antifungal exposure can elicit immunological effects that contribute to activity in vivo, but this activity is rarely screened in vitro in a fashion analogous to MIC testing. We used RAW 264.7 murine macrophages that express a secreted embryonic alkaline phosphatase (SEAP) gene induced by transcriptional activation of NF-κB and activator protein 1 (AP-1) to develop a screen for immunopharmacolo...

Noncanonical Wnt Signaling in Bone

The Wnt family of proteins plays essential roles conserved from flies to human in many developmental processes including cell fate specification, polarity, migration and proliferation. It has becoming increasingly clear that Wnt proteins activate multiple intracellular signaling cascades to achieve the diverse biological functions. One such cascade that has been most intensely studied to date i...

SnapShot: Noncanonical Wnt Signaling Pathways